In situ gelling alginate-pectin blend particles loaded with Ac2-26: A new weapon to improve wound care armamentarium

Pasquale Del Gaudio; Chiara Amante; Roberta Civale; Valentina Bizzarro; Antonello Petrella; Giacomo Pepe; Pietro Campiglia; Paola Russo; Rita P Aquino

doi:10.1016/j.carbpol.2019.115305

In situ gelling alginate-pectin blend particles loaded with Ac2-26: A new weapon to improve wound care armamentarium

Carbohydr Polym. 2020 Jan 1:227:115305. doi: 10.1016/j.carbpol.2019.115305. Epub 2019 Sep 9.

Authors

Pasquale Del Gaudio¹, Chiara Amante², Roberta Civale³, Valentina Bizzarro³, Antonello Petrella³, Giacomo Pepe³, Pietro Campiglia³, Paola Russo³, Rita P Aquino³

Affiliations

¹ Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy. Electronic address: pdelgaudio@unisa.it.
² Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy; PhD Program in Drug Discovery and Development, University of Salerno, via Giovanni Paolo II, 132, I-84084 Fisciano, SA, Italy.
³ Department of Pharmacy, University of Salerno, via Giovanni Paolo II, 132, 84084 Fisciano, SA, Italy.

PMID: 31590879
DOI: 10.1016/j.carbpol.2019.115305

Abstract

In this paper, alginate-pectin blend particles loaded with Annexin A1 derived peptide Ac2-26 as an in situ forming dressing was successfully developed for wound repair applications. High mannuronic (M) content alginate and amidated pectin blend have been used to encapsulate Ac2-26 in order to enhance stability of the peptide at room temperature and to control its release through the in situ formed gel. Ac2-26 recovery and FTIR studies suggests chemical interactions between peptide and polysaccharides blend able to improve the encapsulation efficiency of Ac2-26 into the polymer matrix and control its release, till 48 h. In vitro wound healing assay on HaCaT cells highlights the ability of Ac2-26 to significantly accelerate wound healing compared to unloaded particles, with complete closure of the wound model in 24 h. Therefore, all these results suggest that Ac2-26 loaded submicrometric in situ gelling powders could be a promising wound dressing to improve wound care armamentarium.

Keywords: Alginate; Annexin A1 derived peptide; Controlled release; Pectin; Peptide improved stability; Wound dressing.