Macular thickness measurements of healthy, naïve cynomolgus monkeys assessed with spectral-domain optical coherence tomography (SD-OCT)

Nora Denk; Peter Maloca; Guido Steiner; Christian Freichel; Simon Bassett; Tobias K Schnitzer; Pascal W Hasler

doi:10.1371/journal.pone.0222850

Macular thickness measurements of healthy, naïve cynomolgus monkeys assessed with spectral-domain optical coherence tomography (SD-OCT)

PLoS One. 2019 Oct 7;14(10):e0222850. doi: 10.1371/journal.pone.0222850. eCollection 2019.

Authors

Nora Denk¹, Peter Maloca^{2

3

4}, Guido Steiner¹, Christian Freichel¹, Simon Bassett¹, Tobias K Schnitzer¹, Pascal W Hasler²

Affiliations

¹ Pharma Research and Early Development (pRED), Pharmaceutical Sciences (PS), Roche Innovation Center Basel, Basel, Switzerland.
² OCTlab Research Laboratory, Department of Ophthalmology, University of Basel, Basel, Switzerland.
³ Moorfields Eye Hospital, London, United Kingdom.
⁴ Institute of Molecular and Clinical Ophthalmology Basel (IOB), Basel, Switzerland.

Abstract

The purpose of this study was to measure central macular thickness in an unprecedented number of cynomolgus monkeys. Macular thickness was measured with Heidelberg spectral-domain OCT in 320 eyes of healthy and treatment-naïve cynomolgus monkeys (80 males and 80 females). The macula was successfully measured in all 320 eyes. Macular thickness was not significantly different between the sexes. The mean central macular thickness was 244 μm (+/- 21 μm). Macular thicknesses in the quadrants were 327 +/-17 μm (temporal inner), 339 +/- 17 μm (inferior inner), 341 +/- 14 μm (superior inner), 341 +/-18 μm (nasal inner), and 299 +/- 20 μm (temporal outer), 320 +/- 16 μm (superior outer), 332 +/-23 μm (inferior outer), and 337 +/-18 μm (nasal outer). Highly significant differences between the nasal and temporal quadrants were detected. This study successfully demonstrated the feasibility of retinal thickness measurements in healthy cynomolgus monkeys. The present findings indicate that the macula is thicker in cynomolgus monkeys than in humans and provide important normative data for future studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Macaca fascicularis
Macula Lutea / diagnostic imaging*
Male
Retina / diagnostic imaging
Tomography, Optical Coherence / methods*

Grants and funding

This work was supported by Hoffmann–La Roche Ltd. The funder provided support in the form of salaries for authors ND, GS, CF, SB, TS (employees) as well as PM and PWH (consultants), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.