Decorating protein hydrogels reversibly enables dynamic presentation and release of functional protein ligands on protein hydrogels

Ruidi Wang; Linglan Fu; Junqiu Liu; Hongbin Li

doi:10.1039/c9cc06374a

Decorating protein hydrogels reversibly enables dynamic presentation and release of functional protein ligands on protein hydrogels

Chem Commun (Camb). 2019 Oct 17;55(84):12703-12706. doi: 10.1039/c9cc06374a.

Authors

Ruidi Wang¹, Linglan Fu², Junqiu Liu³, Hongbin Li²

Affiliations

¹ Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada. hongbin@chem.ubc.ca and State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.
² Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada. hongbin@chem.ubc.ca.
³ State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

PMID: 31588464
DOI: 10.1039/c9cc06374a

Abstract

Utilizing protein fragment reconstitution, we demonstrate the reversible and repeatable functionalization of protein hydrogels. This novel method enables the presentation and release of functional protein ligands on protein hydrogels.

MeSH terms

Bacterial Proteins / chemistry*
Cell Line
Cell Survival / drug effects
Cysteine / chemistry
Drug Carriers / chemistry*
Drug Liberation
Fibroblasts / cytology
Green Fluorescent Proteins / chemistry*
Humans
Hydrogels / chemistry*
Ligands
Lung / cytology
Oxidation-Reduction
Peptide Fragments / chemistry*
Protein Binding
Sulfhydryl Compounds / chemistry
Surface Properties
Thermodynamics

Substances

Bacterial Proteins
Drug Carriers
Hydrogels
IgG Fc-binding protein, Streptococcus
Ligands
Peptide Fragments
Sulfhydryl Compounds
enhanced cyan fluorescent protein
Green Fluorescent Proteins
Cysteine