Histological image analysis is becoming an increasingly important tool for research in biological science. They are important in analyzing biological systems on various scales, from structural details to determination of number of cells, its area, localization, and concentration. This chapter focuses on analysis of pancreatic sections stained for insulin and glucagon using a commercially available software.
Keywords: Histology; Image analysis; ImageJ; Immunohistochemistry; Pancreas; VisiomorphDP; Whole-slide image.