sncRNAs packaged by Helicobacter pylori outer membrane vesicles attenuate IL-8 secretion in human cells

Hongxia Zhang; Yingxuan Zhang; Zifan Song; Ruizhen Li; Huan Ruan; Qiong Liu; Xiaotian Huang

doi:10.1016/j.ijmm.2019.151356

sncRNAs packaged by Helicobacter pylori outer membrane vesicles attenuate IL-8 secretion in human cells

Int J Med Microbiol. 2020 Jan;310(1):151356. doi: 10.1016/j.ijmm.2019.151356. Epub 2019 Sep 25.

Authors

Hongxia Zhang¹, Yingxuan Zhang², Zifan Song³, Ruizhen Li⁴, Huan Ruan⁵, Qiong Liu⁶, Xiaotian Huang⁷

Affiliations

¹ Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China. Electronic address: 2063674432@qq.com.
² The First Clinical Medical College, Nanchang University, Nanchang, 330006, China. Electronic address: zyingxuan@outlook.com.
³ The First Clinical Medical College, Nanchang University, Nanchang, 330006, China. Electronic address: kuangyus@126.com.
⁴ The First Clinical Medical College, Nanchang University, Nanchang, 330006, China. Electronic address: eraserli@qq.com.
⁵ Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China. Electronic address: 669597001@qq.com.
⁶ Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China. Electronic address: p19890528@126.com.
⁷ Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China; Key Laboratory of Tumor Pathogenesis and Molecular Pathology, School of Medicine, Nanchang University, Nanchang, 330006, China. Electronic address: xthuang@ncu.edu.cn.

PMID: 31585715
DOI: 10.1016/j.ijmm.2019.151356

Abstract

Bacterial outer membrane vesicles (OMVs) play a vital role in the mechanism of host-pathogen communication, while emerging evidence suggests that OMVs regulate host immune responses through differentially packaged small noncoding RNAs (sncRNAs) to target host mRNA function. Therefore, we identified differentially packaged sncRNAs in Helicobacter pylori OMVs and showed transfer of OMV sncRNAs to human gastric adenocarcinoma cells in this study. Our data revealed that sncRNAs (sR-2509025 and sR-989262) were enriched in OMVs, and reduced lipopolysaccharide or OMV-induced interleukin 8 (IL-8) secretion by cultured AGS cells. Collectively, these findings are consistent with the hypothesis that sncRNAs in H. pylori OMVs play a novel role in the mechanism of host-pathogen interaction, whereby H. pylori evades the host immune response.

Keywords: H.Pylori; IL-8; Outer membrane vesicles; Small noncoding RNAs (sncRNAs).

MeSH terms

Adenocarcinoma
Bacterial Outer Membrane Proteins / genetics*
Cell Line
Cell Line, Tumor
Epithelial Cells / immunology
Epithelial Cells / microbiology
Helicobacter Infections / microbiology
Helicobacter pylori / genetics
Helicobacter pylori / immunology*
Host-Pathogen Interactions / immunology*
Humans
Immune Evasion
Interleukin-8 / immunology*
Protein Transport
RNA, Small Untranslated / immunology*
Stomach Neoplasms

Substances

Bacterial Outer Membrane Proteins
CXCL8 protein, human
Interleukin-8
RNA, Small Untranslated