Plasmid Typing and Classification

Laura Villa; Alessandra Carattoli

doi:10.1007/978-1-4939-9877-7_22

Plasmid Typing and Classification

Methods Mol Biol. 2020:2075:309-321. doi: 10.1007/978-1-4939-9877-7_22.

Authors

Laura Villa¹, Alessandra Carattoli²

Affiliations

¹ Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
² Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy. alessandra.carattoli@uniroma1.it.

PMID: 31584172
DOI: 10.1007/978-1-4939-9877-7_22

Abstract

Plasmids identification and classification is an essential parameter in current bacterial typing. The most widely used PCR-based methods are the PCR-based replicon typing (PBRT) and the degenerate primer MOB typing (DPMT). PBRT targets the replicons on the plasmids and DPMT targets the relaxase genes. A finer resolution of phylogenetic relatedness can be obtained by plasmid multiLocus sequence typing available for the major plasmid types occurring in Enterobacteriaceae.

Keywords: Bacterial typing; DPMT; Incompatibility group; PBRT; pMLST.

MeSH terms

Alleles
Bacterial Typing Techniques
Humans
Molecular Typing*
Multilocus Sequence Typing
Phylogeny
Plasmids / classification*
Plasmids / genetics*
Polymerase Chain Reaction