Rapid thymectomy of NSG mice to analyze the role of native and grafted thymi in humanized mice

Eur J Immunol. 2020 Jan;50(1):138-141. doi: 10.1002/eji.201948205. Epub 2019 Nov 28.

Abstract

We developed a rapid method to remove the native mouse thymus from NSG mice, which allowed us to compare the behavior of human immune cells in the presence or absence of human T cells in human immune system mice. Removing the native mouse thymus is critical for studies of human thymopiesis in grafted thymic tissue in humanized mice.

Keywords: Hematopoietic stem cells; Humanized mice; NSG mice; Thymectomy; Thymopoiesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Thymectomy / methods*
  • Thymus Gland / immunology*
  • Thymus Gland / transplantation*
  • Transplantation, Heterologous / methods*