Study on structure-function of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors: A review

Panpan Guo; Yueqiu Li; Jinxuan An; Shigang Shen; Haiyang Dou

doi:10.1016/j.carbpol.2019.115330

Study on structure-function of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors: A review

Carbohydr Polym. 2019 Dec 15:226:115330. doi: 10.1016/j.carbpol.2019.115330. Epub 2019 Sep 12.

Authors

Panpan Guo¹, Yueqiu Li², Jinxuan An³, Shigang Shen⁴, Haiyang Dou⁵

Affiliations

¹ Key Laboratory of Analytical Science and Technology of Hebei Provine, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China.
² Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases of Hebei Province, College of Medicine, Hebei University, Baoding, 071000, China.
³ School of Science, Royal Melbourne Institute of Technology (RMIT) University, Melbourne, Vic. 3001, Australia.
⁴ Key Laboratory of Analytical Science and Technology of Hebei Provine, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China; Affiliated Hospital of Hebei University, Baoding, 071000, China.
⁵ Key Laboratory of Analytical Science and Technology of Hebei Provine, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China; Affiliated Hospital of Hebei University, Baoding, 071000, China; Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases of Hebei Province, College of Medicine, Hebei University, Baoding, 071000, China. Electronic address: douhaiyang-1984@163.com.

PMID: 31582064
DOI: 10.1016/j.carbpol.2019.115330

Abstract

Starch is one of the most abundant, renewable, non-toxic, and biodegradable polysaccharides, mainly comprising amylose and amylopectin. The physicochemical properties and structure of starch affect the quality of starch-based products. Therefore, the accurate characterization of the physicochemical properties and structure of starch is important to obtain products that meet specific functions. Recently, asymmetrical flow field-flow fractionation coupled with multi-angle light scattering (MALS) and differential refraction detectors (dRI) (AF4-MALS-dRI) has attracted great attention in the separation and characterization of starch. In this review, the structure and functions of starch characterized by AF4-MALS-dRI are summarized. The principle and important characterization parameters of AF4 are introduced. The starch structure-function relationship is deeply investigated owing to utilization of AF4-MALS-dRI. Besides separation of starch, AF4-MALS-dRI can provide more information about starch sample, such as radius of gyration (R_g), hydrodynamic radius (R_h), molar mass (M_w), apparent density (ρ), and conformation (R_g/R_h) of starch molecules. These parameters can be further used to investigate the structure-function relationship of starch.

Keywords: Asymmetrical flow field-flow fractionation; Function; Starch; Structure.

Publication types

Review