Aim: Change at a molecular level should occur before histopathological impairments. We refer to these changes as 'prechronic disease state.' Methods: Two blood samples were taken 1 day apart, 1 month apart and 2 years apart to determine the contribution of genetic and biological variations. Results: The 95% CI calculated quantitatively by the variability of genetic variation was very similar to the reference interval provided by the laboratory for most indicators, indicating that pathological variability had occurred without consideration of biological variation if an indicator's value lies outside of the reference interval. Conclusion: The physiological or predisease state can be distinguished directly using the laboratory reference interval.
Keywords: biological variation; biomarker; blood; chronic disease; health; ill health; medical laboratory; nonalcoholic fatty liver; prechronic disease; reference range.