MALDI-TOF Mass Spectrometric Profiling of Spider Venoms

Ondrej Šedo; Stano Pekár; Zbyněk Zdráhal

doi:10.1007/978-1-4939-9845-6_9

MALDI-TOF Mass Spectrometric Profiling of Spider Venoms

Methods Mol Biol. 2020:2068:173-181. doi: 10.1007/978-1-4939-9845-6_9.

Authors

Ondrej Šedo¹, Stano Pekár², Zbyněk Zdráhal^{3

4}

Affiliations

¹ Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
² Faculty of Science, Department of Botany and Zoology, Masaryk University, Brno, Czech Republic.
³ Central European Institute of Technology, Masaryk University, Brno, Czech Republic. zdrahal@sci.muni.cz.
⁴ Faculty of Science, National Centre for Biomolecular Research, Masaryk University, Brno, Czech Republic. zdrahal@sci.muni.cz.

PMID: 31576528
DOI: 10.1007/978-1-4939-9845-6_9

Abstract

Fingerprinting by means of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) represents a tool for rapidly detecting proteinaceous compounds from spider venoms. Here we describe an optimized protocol and discuss methodological details with the aim of providing a platform for obtaining the most informative and reproducible mass spectral data.

Keywords: Fingerprinting; MALDI-TOF MS; Proteins; Spider venom.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Proteins / analysis
Proteins / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Spider Venoms / analysis*
Spider Venoms / chemistry

Substances

Proteins
Spider Venoms