Determining the Structures of the Snake and Spider Toxins by X-Rays

Methods Mol Biol. 2020:2068:163-172. doi: 10.1007/978-1-4939-9845-6_8.

Abstract

Snake and spider venom is a complex mixture that contains proteins, peptides, and small organic and inorganic compounds. In contrast to spider venom, snake venom proteins are well known both functionally and structurally. This work describes methods for purification and crystallization of snake and spider venom toxins and their three-dimensional structure determination by X-ray crystallography.

Keywords: Crystallization; Purification; Snake and spider venom toxins; Three-dimensional structure determination.

MeSH terms

  • Animals
  • Crystallography, X-Ray / methods*
  • Peptides / analysis
  • Proteins / analysis
  • Snake Venoms / analysis*
  • Spider Venoms / analysis*

Substances

  • Peptides
  • Proteins
  • Snake Venoms
  • Spider Venoms