1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector

A Tolstikova; M Levantino; O Yefanov; V Hennicke; P Fischer; J Meyer; A Mozzanica; S Redford; E Crosas; N L Opara; M Barthelmess; J Lieske; D Oberthuer; E Wator; I Mohacsi; M Wulff; B Schmitt; H N Chapman; A Meents

doi:10.1107/S205225251900914X

1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector

IUCrJ. 2019 Aug 17;6(Pt 5):927-937. doi: 10.1107/S205225251900914X. eCollection 2019 Sep 1.

Authors

A Tolstikova^{1

2}, M Levantino³, O Yefanov¹, V Hennicke¹, P Fischer¹, J Meyer⁴, A Mozzanica⁵, S Redford⁵, E Crosas⁴, N L Opara^{5

6}, M Barthelmess¹, J Lieske¹, D Oberthuer¹, E Wator⁷, I Mohacsi¹, M Wulff³, B Schmitt⁵, H N Chapman^{1

2

8}, A Meents^{1

4}

Affiliations

¹ Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
² Department of Physics, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
³ European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France.
⁴ Deutsches Elektronen Synchrotron, Photon Science, Notkestrasse 85, 22607 Hamburg, Germany.
⁵ Paul Scherrer Institute, 111 Forschungsstrasse, 5232 Villigen, Switzerland.
⁶ C-CINA, Biozentrum, University of Basel, Mattenstrasse 26, 4002 Basel, Switzerland.
⁷ Malopolska Centre of Biotechnology, Jagiellonian University, Cracow 30-387, Poland.
⁸ Centre for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, Hamburg 22761, Germany.

Abstract

Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.

Keywords: pink beams; protein crystallography; protein structure; serial crystallography; structure determination; synchrotron radiation.

Grants and funding

This work was funded by H2020 Marie Skłodowska-Curie Actions grant 637295. Horizon 2020 grant 654220. H2020 European Research Council grant 609920. Bundesministerium für Bildung und Forschung grants 05K2018-2017-06727 and 031B0405D. Swiss Nanoscience Institute grant P1305.