Myoglobin- and Hydroxyapatite-Doped Carbon Nanofiber-Modified Electrodes for Electrochemistry and Electrocatalysis

Juan Liu; Wenju Weng; Hui Xie; Guiling Luo; Guangjiu Li; Wei Sun; Chengxiang Ruan; Xianghui Wang

doi:10.1021/acsomega.9b02151

Myoglobin- and Hydroxyapatite-Doped Carbon Nanofiber-Modified Electrodes for Electrochemistry and Electrocatalysis

ACS Omega. 2019 Sep 11;4(13):15653-15659. doi: 10.1021/acsomega.9b02151. eCollection 2019 Sep 24.

Authors

Juan Liu^{1

2}, Wenju Weng², Hui Xie¹, Guiling Luo¹, Guangjiu Li², Wei Sun¹, Chengxiang Ruan³, Xianghui Wang¹

Affiliations

¹ Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China.
² Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
³ Key Laboratory of Surface Engineering of Jiangxi Province, Jiangxi Science and Technology Normal University, Nanchang 330013, P. R. China.

Abstract

In this paper, a hydroxyapatite (HAp)-doped carbon nanofiber (CNF)-modified carbon ionic liquid electrode (CILE) was prepared and used for the investigation on the direct electrochemistry and electrocatalysis of myoglobin (Mb). HAp nanoparticles were mixed within a polyacrylonitrile (PAN) solution, and a HAp@PAN nanofiber was synthesized by electrospinning process, which was further controlled by carbonization at 800 °C for 2 h in a nitrogen atmosphere to get a HAp@CNF nanocomposite. Various techniques were used to check the physicochemical properties of HAp@CNF. Mb was mixed with a HAp@CNF dispersion solution and casted on the surface of CILE to obtain an electrochemical sensing platform. The direct electrochemistry of Mb on the modified electrode was checked when a pair of enhanced redox waves appeared, indicating the direct electron transfer of Mb. HAp@CNF exhibited high conductivity, good biocompatibility, and large surface area, which was beneficial for Mb immobilization. The modified electrode showed excellent electrocatalytic activity toward the reduction of trichloroacetic acid and sodium nitrite, which was further used to establish a new electroanalytical method. Real samples were analyzed by the proposed method with satisfactory results.