Nitrate stimulation of N-Methylpyrrolidone biodegradation by Paracoccus pantotrophus: Metabolite mechanism and Genomic characterization

Jing Wang; Xiaolin Liu; Xinbai Jiang; Libin Zhang; Cheng Hou; Guanyong Su; Lianjun Wang; Yang Mu; Jinyou Shen

doi:10.1016/j.biortech.2019.122185

Nitrate stimulation of N-Methylpyrrolidone biodegradation by Paracoccus pantotrophus: Metabolite mechanism and Genomic characterization

Bioresour Technol. 2019 Dec:294:122185. doi: 10.1016/j.biortech.2019.122185. Epub 2019 Sep 24.

Authors

Jing Wang¹, Xiaolin Liu¹, Xinbai Jiang¹, Libin Zhang², Cheng Hou¹, Guanyong Su¹, Lianjun Wang¹, Yang Mu³, Jinyou Shen⁴

Affiliations

¹ Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
² Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: lbzhang@njust.edu.cn.
³ CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, China.
⁴ Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: shenjinyou@mail.njust.edu.cn.

PMID: 31569046
DOI: 10.1016/j.biortech.2019.122185

Abstract

Due to the toxicological nature of N-methylpyrrolidone (NMP), the conventional anaerobic bioprocess is quite ineffective for NMP removal from wastewater. In order to achieve effective NMP biodegradation under anoxic condition, Paracoccus pantotrophus NJUST38 was isolated for the first time. The supplementation of nitrate into anoxic system resulted in complete removal of 5 mM NMP by NJUST38 within 11 h compared to 24% in the anaerobic control system in the absence of nitrate. Genome characterization revealed that NMP biodegradation catalyzed by several key enzymes/genes, including N-methylhydantoin amidohydrolase (hyuB), methyltransferase (cobA), 4-aminobutyrate-2-oxoglutarate transaminase (gabT), succinate-semialdehyde dehydrogenase (gabD) and so on. NMP biodegradation pathway was proposed based on several intermediates, where NMP was biodegraded mainly for providing electrons and reducing power to support microbial denitrification through tricarboxylic acid (TCA) cycle. The proposed mechanism should aid our mechanistic understanding of NMP biodegradation by Paracoccus pantotrophus and the development of sustainable bioremediation strategies.

Keywords: Biodegradation; Denitrification; Functional genes; N-Methylpyrrolidone; Paracoccus.

MeSH terms

Biodegradation, Environmental
Genomics
Nitrates
Paracoccus pantotrophus*
Paracoccus*
Pyrrolidinones

Substances

Nitrates
Pyrrolidinones
N-methylpyrrolidone