Abstract
We isolated Lactobacillus mucosae NK41 and Bifidobacterium longum NK46 from human feces, which induced BDNF expression in corticosterone-stimulated SH-SY5Y cells, and examined their anti-depressive effects in mice. NK41, NK46, and their (1:1) mixture significantly mitigated immobilization stress (IS)-induced anxiety-like/depressive behaviors, hippocampal NF-κB activation, BDNF expression, Iba1+ cell population, and blood corticosterone, TNF-α, IL- 6, and lipopolysaccharide levels. Furthermore, they inhibited colitis marker NF-κB activation, and TNF-α expression in mice with IS-induced anxiety/depression. They additionally suppressed gut Proteobacteria and Bacteroidetes populations and bacterial lipopolysaccharide production. These findings suggest that NK41 and NK46 may alleviate anxiety/depression and colitis by suppressing gut dysbiosis.
Keywords:
Bifidobacterium longum; Lactobacillus mucosae; anxiety; depression; gut microbiota.
MeSH terms
-
Animals
-
Antidepressive Agents / administration & dosage
-
Antidepressive Agents / pharmacology
-
Antidepressive Agents / therapeutic use
-
Anxiety / blood
-
Anxiety / diet therapy*
-
Anxiety / microbiology
-
Bifidobacterium longum*
-
Brain-Derived Neurotrophic Factor / metabolism
-
Calcium-Binding Proteins / metabolism
-
Colitis / microbiology
-
Colitis / pathology
-
Corticosterone / blood
-
Depression / blood
-
Depression / diet therapy*
-
Depression / microbiology
-
Disease Models, Animal
-
Dysbiosis / microbiology
-
Dysbiosis / pathology
-
Feces / microbiology
-
Gastrointestinal Microbiome / drug effects*
-
Hippocampus / drug effects
-
Hippocampus / metabolism
-
Humans
-
Interleukin-6 / blood
-
Lactobacillus*
-
Lipopolysaccharides / metabolism
-
Male
-
Mice, Inbred C57BL
-
Microfilament Proteins / metabolism
-
NF-kappa B / metabolism
-
Probiotics / administration & dosage
-
Probiotics / pharmacology*
-
Probiotics / therapeutic use*
-
Stress, Psychological / blood
-
Stress, Psychological / microbiology
-
Stress, Psychological / psychology
-
Tumor Necrosis Factor-alpha / blood
Substances
-
Aif1 protein, mouse
-
Antidepressive Agents
-
Brain-Derived Neurotrophic Factor
-
Calcium-Binding Proteins
-
Interleukin-6
-
Lipopolysaccharides
-
Microfilament Proteins
-
NF-kappa B
-
Tumor Necrosis Factor-alpha
-
Corticosterone