In vivo ratiometric tracking of endogenous β-galactosidase activity using an activatable near-infrared fluorescent probe

Chem Commun (Camb). 2019 Oct 10;55(82):12308-12311. doi: 10.1039/c9cc06869d.

Abstract

Herein, we developed a dual-channel and light-up near-infrared fluorescent probe for ratiometric sensing of β-galactosidase (β-gal) activity. The well-designed probe, which shows ratiometric optical response with a significant red-shift (from 575 nm to 730 nm), was successfully applied to detect endogenous β-gal activity in SKOV-3 cells and tumor-bearing mice.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry*
  • Hep G2 Cells
  • Humans
  • Infrared Rays
  • Mice
  • Mice, Nude
  • Molecular Structure
  • Neoplasms, Experimental / diagnostic imaging
  • Optical Imaging
  • beta-Galactosidase / analysis*
  • beta-Galactosidase / metabolism*

Substances

  • Fluorescent Dyes
  • beta-Galactosidase