Authentication Analysis of MT-4 Cells Distributed by the National Institutes of Health AIDS Reagent Program

J Virol. 2019 Nov 26;93(24):e01390-19. doi: 10.1128/JVI.01390-19. Print 2019 Dec 15.

Abstract

The MT-4 human T-cell line expresses HTLV-1 Tax and is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. MT-4 cells (lot 150048), distributed by the NIH AIDS Reagent Program (NIH-ARP), were found to be Tax deficient and unable to host replication of the gp41-truncated HIV-1 mutant. These findings, together with short tandem repeat profiling, established that lot 150048 are not bona fide MT-4 cells.

Keywords: MT-4; STR profiling; authentication; short tandem repeat.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Acquired Immunodeficiency Syndrome*
  • Cell Line / virology*
  • Gene Products, tax / genetics
  • HIV Envelope Protein gp41 / genetics
  • HIV-1 / genetics
  • Human T-lymphotropic virus 1
  • Humans
  • Microsatellite Repeats
  • National Institutes of Health (U.S.)
  • T-Lymphocytes / virology*
  • United States
  • Virus Replication

Substances

  • Gene Products, tax
  • HIV Envelope Protein gp41
  • gp41 protein, Human immunodeficiency virus 1