A Facile and Universal Method to Efficiently Fabricate Diverse Protein Capsules for Multiple Potential Applications

Pengzhong Shi; Jianghui Qin; Xiaoxiao Wu; Liwen Wang; Tinghong Zhang; Dejun Yang; Xingjie Zan; Dietmar Appelhans

doi:10.1021/acsami.9b15019

A Facile and Universal Method to Efficiently Fabricate Diverse Protein Capsules for Multiple Potential Applications

ACS Appl Mater Interfaces. 2019 Oct 23;11(42):39209-39218. doi: 10.1021/acsami.9b15019. Epub 2019 Oct 8.

Authors

Pengzhong Shi^{1

2

3}, Jianghui Qin¹, Xiaoxiao Wu¹, Liwen Wang¹, Tinghong Zhang^{2

3}, Dejun Yang^{2

3}, Xingjie Zan^{1

2

3}, Dietmar Appelhans⁴

Affiliations

¹ School of Ophthalmology and Optometry, Eye Hospital, School of Biomedical Engineering , Wenzhou Medical University , Wenzhou , Zhejiang Province 325035 , P. R. China.
² Wenzhou Institute of Biomaterials and Engineering, Wenzhou Institute , University of Chinese Academy of Sciences , Wenzhou , Zhejiang Province 325001 , P. R. China.
³ Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute of Biomaterials and Engineering, Wenzhou Institute , University of Chinese Academy of Sciences , Wenzhou , Zhejiang Province 325001 , P. R. China.
⁴ Leibniz Institute of Polymer Research Dresden , Hohe Strasse 6 , Dresden 01069 , Germany.

PMID: 31553877
DOI: 10.1021/acsami.9b15019

Abstract

Proteins are considered to be one of the most important highly reproducible and monodisperse building blocks with specific functions in life sciences and material science. Protein capsules and their hybrids composed of protein-polymer conjugates have been intensively explored in drug delivery, catalysis, and cell-mimicking functions. Herein, we present a facile, universal, and efficient method to fabricate the diverse protein capsules, independent of the molecular weight (M_w), isoelectric points (IEP), wettability, amino acid sequence, and functional domains of enumerated proteins. The protein capsules were well characterized by various techniques. Furthermore, their ability to store the original protein functionality was demonstrated, which was mainly embodied in their enzyme responsiveness and good biocompatibility in vitro and in vivo. We believe that these protein capsules have multiple potential applications such as in drug delivery, tissue engineering, catalysis, and other application fields.

Keywords: CaCO3; capsules; catalysis; protein; tannic acid.