The rapid and sensitive identification of bacteria has long been a major challenge in quality control, environmental monitoring and food safety. In the current study, the "motion-capture-lighting" strategy is proposed via integration of motion-enhanced capture of bacteria and capture-induced fluorescence turn-on of micromotors. Compared with the commonly used microtubes and microparticles, micromotors of flexible fiber rods could offer multiple interactions with the bacterial surface with less steric hindrance. Janus fiber rods (JFRs) are prepared by cryocutting of aligned fibers prepared by side-by-side electrospinning. Catalase is grafted on one side of JFRs to produce oxygen bubbles for propulsion of Janus micromotors (JMs), and mannose is conjugated on the other side for specific recognition of FimH proteins from fimbriae on the bacterial surface. The biphasic Janus structure of JFRs and the separate grafting of catalase and mannose on the opposite sides of JMs are confirmed after fluorescent labelling. JMs with aspect ratios of 0.5, 1, 2 and 4 are fabricated, and the aspect ratios of JMs show significant effects on the tracking trajectories and motion speed. JMs with the aspect ratio of 2 exhibit significantly higher magnitudes of mean square displacement (MSD) with a directional motion trajectory, leading to higher bacterial capture and larger fluorescence intensity changes. The bacteria capture leads to lighting up of JMs due to the aggregation-induced emission (AIE) effect of tetraphenylethene (TPE) derivatives. Under an ultraviolet lamp, the fluorescence color of JM suspensions turns from blue to bluish-green and to green after incubation with E. coli of 102 and 105 CFU mL-1, respectively. The fluorescence intensities of JM suspensions could be fitted to an equation versus bacterial concentrations, and the limit of detection (LOD) was around 45 CFU mL-1 within 1 min. Thus, this study demonstrates a motion-capture-lighting strategy for visual, rapid and real-time detection of bacteria without complicated sample pretreatment, expensive apparatus, and trained operators.