Shotgun proteomics is the method of choice for large-scale protein identification. However, the use of a robust statistical workflow to validate such identification is mandatory to minimize false matches, ambiguities, and amplification of error rates from spectra to proteins. In this chapter we emphasize the key concepts to take into account when processing the output of a search engine to obtain reliable peptide or protein identifications. We assume that the reader is already familiar with tandem mass spectrometry so we can focus on the use of statistical confidence methods. After introducing the key concepts we present different software tools and how to use them with an example dataset.
Keywords: Bioinformatics; False discovery rate; Peptide identification; Protein inference; Shotgun proteomics; Target–decoy approach.