Self-Assembled Two-Dimensional Molybdenum Disulfide Nanosheet Geno-Interface for the Detection of Salmonella

Annu Mishra; Tarab Fatima; Jagriti Narang; Sudheesh K Shukla; Rachna Rawal; Ashish Mathur; Akshay Jain; Manika Khanuja

doi:10.1021/acsomega.9b01651

Self-Assembled Two-Dimensional Molybdenum Disulfide Nanosheet Geno-Interface for the Detection of Salmonella

ACS Omega. 2019 Sep 3;4(12):14913-14919. doi: 10.1021/acsomega.9b01651. eCollection 2019 Sep 17.

Authors

Annu Mishra¹, Tarab Fatima¹, Jagriti Narang², Sudheesh K Shukla^{3

4}, Rachna Rawal⁵, Ashish Mathur¹, Akshay Jain¹, Manika Khanuja⁶

Affiliations

¹ Amity Institute of Nanotechnology, Amity University, Noida 201313, UP, India.
² Department of Biotechnology, Jamia Hamdard, New Delhi 110062, UP, India.
³ Department of Applied Chemistry, University of Johannesburg, P.O. Box 17011, Doornfontein Campus, Johannesburg 2028, South Africa.
⁴ School of Environmental Science and Engineering, Shandong University, Jimo, Qingdao 266237, P.R. China.
⁵ Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
⁶ Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia, New Delhi 110025, India.

Abstract

This report presents a novel lab-on-a-paper (LoP)-based device coupled with a molybdenum disulfide nanosheet (MoS₂NS)-modified electrochemical genosensor for detecting Salmonella-specific DNA. Conductive electrodes were grafted on a paper-based substrate employing a stencil printing technique, and MoS₂NS was decorated on the working electrode. MoS₂NS has strong affinity toward nucleo bases, which made it a best sensing interface for the immobilization of DNA. Morphological, optical, and structural characterizations were accomplished using X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), UV-vis spectroscopy (UV-vis), and Raman spectroscopy, repectively. The current studies of an electrochemical genosensor demonstrated a good linear detection range from 100-20 nM and a low limit of detection of 20 nM toward Salmonella DNA with R ² = 0.991. The proposed LoP-based genosensor confirmed as a better sensing podium and an effectual immobilization matrix for DNA.