Sludge bulking is a major problem that restricts the development of the activated sludge process. The microbial community responsible for sludge bulking varies depending on water quality and operational conditions. This study analysed the microbial community of bulking sludge in oxidation ditch with ultra-low sludge loading and long sludge retention time using high-throughput sequencing. The study found that the relative abundance of bacterial genus Saprospiraceae_norank was the highest in bulking sludge, reaching 13.39-28.83%, followed by Comamonadaceae_unclassified, Ardenticatenia_norank and Tetrasphaera, with the relative abundance of 4.59-11.08%, 0.52-16.60% and 0.17-8.92% respectively. In contrast, the relative abundance of bacteria that easily caused sludge bulking including Microthrix (0.54-2.47%), Trichococcus (0.32-1.71%), Gordonia (0.14-1.28%), and Thiothrix (0.01-0.06%) were relatively low. Saprospiraceae_norank was predominant and induced sludge bulking in oxidation ditch. The relative abundance of fungal genus Trichosporon was the highest in bulking sludge, reaching 16.95-24.98%, while other fungal genera were Saccharomycetales_unclassified (5.59-14.55%), Ascomycota_norank (1.45-13.51%), Galactomyces (5.23-11.23%), and Debaryomyces (7.69-9.42%), whereas Trichosporon was the dominant fungal genus in bulking sludge. This study reported that excessive Saprospiraceae_norank can induce sludge bulking for the first time, which provides important knowledge to control sludge bulking.