One pot facile green synthesis of crystalline bio-ZrO2 nanoparticles using Acinetobacter sp. KCSI1 under room temperature

Shanmugasundaram Prema Suriyaraj; Govindarajan Ramadoss; Krishnan Chandraraj; Rajendran Selvakumar

doi:10.1016/j.msec.2019.110021

One pot facile green synthesis of crystalline bio-ZrO₂ nanoparticles using Acinetobacter sp. KCSI1 under room temperature

Mater Sci Eng C Mater Biol Appl. 2019 Dec:105:110021. doi: 10.1016/j.msec.2019.110021. Epub 2019 Jul 26.

Authors

Shanmugasundaram Prema Suriyaraj¹, Govindarajan Ramadoss², Krishnan Chandraraj³, Rajendran Selvakumar⁴

Affiliations

¹ Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India; PSG-STEP: Nanotech Research Innovation and Incubation centre (NRIIC), PSG College of Technology Coimbatore, 641004, India.
² Nanobiotechnology Laboratory, PSG Institute of Advanced Studies, Coimbatore 641004,India; Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 602105, India.
³ Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India. Electronic address: kcraj@iitm.ac.in.
⁴ Nanobiotechnology Laboratory, PSG Institute of Advanced Studies, Coimbatore 641004,India. Electronic address: rsk@psgias.ac.in.

PMID: 31546379
DOI: 10.1016/j.msec.2019.110021

Abstract

In this study, crystalline ZrO₂ nanoparticles were synthesized in one pot at room temperature using an extremophilic Acinetobacter sp. KCSI1 and characterized using various techniques to study its structural, optical and crystalline properties. The average size of the ZrO₂ nanoparticles was found to be 44 ± 7 nm. The XRD and Raman spectra showed the crystalline structure of ZrO_2. HRTEM and SAED images showed well aligned ordered crystal lattice nanoparticles. The zeta potential of Bio-NP of ZrO₂ has been found to be 36.5 ± 5.46 mV. The mechanical behaviour such as hardness and Young's modulus of Bio-ZrO₂ NPs were determined using atomic force microscopy (AFM) and found to be 9.206 ± 2.22 and 0.285 ± 0.13 GPa, respectively. No significant cytotoxicity for ZrO₂ nanoparticles was observed when tested with mouse fibroblast cells (L929), suggesting that the synthesized ZrO₂ nanoparticle is biocompatible and safe for environmental applications.

Keywords: Acinetobacter; Biosynthesis; Cytotoxicity; ZrO(2) nanoparticles.

MeSH terms

Acinetobacter / metabolism*
Acinetobacter / ultrastructure
Animals
Cell Death
Cell Line
Cell Survival
Crystallization
DNA / metabolism
Green Chemistry Technology / methods*
Mice
Nanoparticles / chemistry*
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Temperature*
X-Ray Diffraction
Zirconium / chemistry*

Substances

DNA
Zirconium
zirconium oxide