Bio-functional G-molecular hydrogels for accelerated wound healing

Mingshu Xiao; Lihu Gao; Arun Richard Chandrasekaran; Jianfeng Zhao; Qian Tang; Zhibei Qu; Fei Wang; Li Li; Yang Yang; Xueli Zhang; Ying Wan; Hao Pei

doi:10.1016/j.msec.2019.110067

Bio-functional G-molecular hydrogels for accelerated wound healing

Mater Sci Eng C Mater Biol Appl. 2019 Dec:105:110067. doi: 10.1016/j.msec.2019.110067. Epub 2019 Aug 9.

Authors

Mingshu Xiao¹, Lihu Gao², Arun Richard Chandrasekaran³, Jianfeng Zhao⁴, Qian Tang¹, Zhibei Qu⁵, Fei Wang⁵, Li Li¹, Yang Yang⁶, Xueli Zhang⁷, Ying Wan⁸, Hao Pei⁹

Affiliations

¹ Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
² School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Jiangsu JLand Biotech. Co., Ltd., 49 Yongxing Street, Jingjiang 214500, Jiangsu, China.
³ The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, United States.
⁴ Jiangsu JLand Biotech. Co., Ltd., 49 Yongxing Street, Jingjiang 214500, Jiangsu, China.
⁵ Joint Research Center for Precision Medicine, Shanghai Jiao Tong University & Affiliated Sixth People's Hospital South Campus, 6600th Nanfeng Road, Fengxian District, Shanghai 201499, China.
⁶ Bioengineering Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
⁷ Joint Research Center for Precision Medicine, Shanghai Jiao Tong University & Affiliated Sixth People's Hospital South Campus, 6600th Nanfeng Road, Fengxian District, Shanghai 201499, China; Southern Medical University Affiliated Fengxian Hospital, Shanghai 201499, China. Electronic address: lejing1996@aliyun.com.
⁸ School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: wanying@njust.edu.cn.
⁹ Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China. Electronic address: peihao@chem.ecnu.edu.cn.

PMID: 31546352
DOI: 10.1016/j.msec.2019.110067

Abstract

Development of biomedical materials for proper collagen deposition is of great importance to accelerate wound healing and thus achieving skin regeneration. Here, we report guanosine quartet hydrogels loaded with recombinant human-source collagen (G4-RHC) that can be used as medical patches for wound repair. The G4-RHC hydrogels are flexible, and when wrapped onto the skin surface, supplies proper RHC deposition for the wound. We demonstrate the efficiency of the hydrogels through in vitro assays, in vivo wound healing mouse models and histological analysis. G4-RHC hydrogels promote wound healing and facilitate skin generation due to the ability of the deposited RHC to recruit macrophages and fibroblasts to the wound site and induce their proliferation and migration. Given these features of this flexible material, the synthesized G4-RHC hydrogels hold great potential in biomedical applications involving tissue regeneration.

Keywords: Flexible materials; Hydrogel; Medical patch; RHC; Wound healing.

MeSH terms

Animals
Cell Line
Collagen* / chemistry
Collagen* / pharmacology
Fibroblasts / metabolism*
Fibroblasts / pathology
Hydrogels* / chemistry
Hydrogels* / pharmacology
Macrophages / metabolism*
Macrophages / pathology
Mice
Recombinant Proteins / chemistry
Recombinant Proteins / pharmacology
Wound Healing / drug effects*

Substances

Hydrogels
Recombinant Proteins
Collagen