Proliferative Effect of Tilapia Fish (Oreochromis niloticus) Lectin on BALB/c Mice Splenocytes

Protein Pept Lett. 2019;26(12):887-892. doi: 10.2174/0929866526666190911144057.

Abstract

Background: Lectins have been studied in recent years due to their immunomodulatory activities.

Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes.

Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis.

Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines.

Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.

Keywords: Oreochromis niloticus; cytokines; immunostimulation; lectin; splenocytes; tilapia fish..

MeSH terms

  • Animals
  • Cell Death
  • Cell Line
  • Cell Proliferation / drug effects*
  • Cell Survival
  • Concanavalin A / pharmacology
  • Cytokines / biosynthesis
  • Male
  • Mannose-Binding Lectins / pharmacology*
  • Mice, Inbred BALB C
  • Mitogens / pharmacology*
  • Spleen / cytology*
  • Tilapia

Substances

  • Cytokines
  • Mannose-Binding Lectins
  • Mitogens
  • Concanavalin A