Objective: To understand Clonorchis sinensis infections in cats in Nanning City, so as to provide evidence for the control of the reservoir host of C. sinensis.
Methods: The cat livers were purchased from cat slaughterhouses in Nanning City. The cat gallbladder and liver were dissected, and liver flukes were collected and counted. Then, the worms were subjected to morphological observation, amplification of the ITS2 gene and sequencing. The species of the worms were identified using BLAST.
Results: A total of 105 cat livers were collected from two cat slaughterhouses, and 68 were detected with C. sinensis infections, with an infection rate of 64.76%. The highest burden was 980 worms in a single liver, and the mean burden was 72 worms in a liver. There were 3 types of liver flukes with various size and morphology, and all were identified as C. sinensis by means of morphological observation, ITS2 gene amplification, sequencing and sequence alignment.
Conclusions: There is a high infection rate of C. sinensi in marketed cats in Nanning City, and it is therefore suggested that targeted interventions should be intensified for the management of C. sinensis infections in cats.
[摘要] 目的 了解南宁市猫华支睾吸虫感染情况, 为华支睾吸虫保虫宿主防控提供依据。方法 从南宁市猫屠宰点 购买猫肝, 解剖胆囊和肝脏, 查找华支睾吸虫并计数; 对收集到的不同形态和大小的华支睾吸虫进行形态学观察和 ITS2 基因扩增和测序, 通过 BLAST 比对分析确定虫体种类。结果 从南宁市 2 个猫屠宰摊面共收集了 105 个猫肝脏, 其中有 68 个发现有华支睾吸虫感染, 感染率为 64.76%。单个肝脏虫体数量最多达 980 条, 中位数为 72 条。从检出的虫体中分 离到 3 种不同大小和形态的吸虫, 经形态学和 ITS2 基因扩增、测序、比对分析鉴定, 均为华支睾吸虫。结论 南宁市市 售猫华支睾吸虫感染率较高, 应该加强相关防控措施。.
Keywords: Cat; Clonorchis sinensis; ITS2 gene; Nanning City; Reservoir host.