To confirm the expression of HLA-DR antigen on the thyrocytes of Graves’ disease patients and study the relation between the degree of DR antigen expression and clinical or laboratory indices, double immunofluorescence and immunoenzymatic staining were performed on frozen thyroid sections. DR antigen was expressed on the thyrocytes of 8 of 11 patients with Graves’ disease (73%) but not found on the thyrocytes from 6 normal controls. The degree of DR antigen expression had no apparent correlation with the age, duration of the disease, activity of TBII, titers of antimicrosomal and antithyroglobulin antibody. There was no apparent spatial or quantitative relation between lymphoid follicle formation and the DR antigen expression on the thyroid follicles. But DR antigen tended to be expressed on the thyroid tissue with interstitial, lymphocyte infiltration. In conclusion, DR antigen was expressed on the thyrocytes of Graves’ disease patients but the clinical and immunological significance remains to be clarified.