Dysregulated host immune homeostasis in sepsis is life-threatening even after a successfully treated bacterial infection. Lipopolysaccharide (LPS) is an endotoxin that is a major contributor to the aberrant immune responses and endotoxic shock in gram-negative bacterial sepsis. However, the current knowledge of the role of B cells in endotoxic shock is limited. Here, we report that CD1d expression in B cells and the percentage of CD5+CD1dhi regulatory B (Breg) cells decreased in a mouse model of endotoxic shock. Interestingly, IL-10 but not FasL expression in CD5+CD1dhi Breg cells in response to endotoxin was dramatically reduced in severe septic shock mice, and the regulatory function of CD5+CD1dhi Breg cells in vitro to control the Th1 response was also diminished. Adoptive transfer of CD5+CD1dhi Breg cells from healthy WT mice but not IL-10 deficient mice downregulated the IFN-γ secretion in CD4+ T cells and conferred protection against severe endotoxic shock in vivo. Our findings demonstrate the change and notable therapeutic potential of IL-10-producing Breg cells in endotoxic shock.
Keywords: 5(6)-CFDA N-succinimidyl ester (CFSE, PubChem, CID: 71314542); CD1d; CD4(+) T cells; Endotoxic shock; Eosin (PubChem CID: 11048); Haematoxylin (PubChem CID: 442514); IL-10; Ionomycin (PubChem CID: 6912226); Lipopolysaccharide (PubChem CID: 11970143); Monensin (PubChem CID: 441145); Phorbol 12-myristate 13-acetate (PMA, PubChem CID: 27924); Regulatory B cells.
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