Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum

Arnaud T Djami-Tchatchou; Lerato B T Matsaunyane; Khayalethu Ntushelo

doi:10.1002/mbo3.911

Gene expression responses of tomato inoculated with Pectobacterium carotovorum subsp. carotovorum

Microbiologyopen. 2019 Dec;8(12):e911. doi: 10.1002/mbo3.911. Epub 2019 Sep 19.

Authors

Arnaud T Djami-Tchatchou¹, Lerato B T Matsaunyane², Khayalethu Ntushelo¹

Affiliations

¹ Department of Agriculture and Animal Health, Science Campus, University of South Africa, Florida, South Africa.
² Agricultural Research Council-Vegetable and Ornamental Plants, Tshwane, South Africa.

Abstract

Defense responses of tomato (Solanum lycopersicum L.) against attack by Pectobacterium carotovorum subsp. carotovorum (Pcc), the causal agent of soft rot diseases, were studied. The expression of some tomato defense genes were evaluated by real-time PCR quantification analysis, 24 and 72 hr after actively growing tomato plants were inoculated with Pcc. These included: MYB transcriptor factor, ethylene response element-binding protein, suppressor of the G2 allele of Skp1, cytochrome P450, small Sar1 GTPase, hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase, pathogenesis-related protein 1a, endo-1,3-beta-glucanase, chitinase, proteinase inhibitor, defensin, CC-NBS-LRR resistance protein, and phenylalanine ammonia lyase. The results showed dynamic transcriptomic changes, with transcripts exhibiting different expression kinetics at 24 and 72 hr to confer resistance to tomato against Pcc infection.

Keywords: Pectobacterium carotovorum subsp. carotovorum; defense; gene expression; tomato.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling
Gene Expression Regulation, Plant*
Host-Pathogen Interactions / genetics*
Pectobacterium carotovorum*
Plant Diseases / genetics*
Plant Diseases / microbiology*
Solanum lycopersicum / genetics*
Solanum lycopersicum / microbiology*
Transcriptome