Surface Plasmon Resonance Microscopy: From Single-Molecule Sensing to Single-Cell Imaging

Xiao-Li Zhou; Yunze Yang; Shaopeng Wang; Xian-Wei Liu

doi:10.1002/anie.201908806

Surface Plasmon Resonance Microscopy: From Single-Molecule Sensing to Single-Cell Imaging

Angew Chem Int Ed Engl. 2020 Jan 27;59(5):1776-1785. doi: 10.1002/anie.201908806. Epub 2019 Oct 18.

Authors

Xiao-Li Zhou¹, Yunze Yang², Shaopeng Wang², Xian-Wei Liu¹

Affiliations

¹ CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science & Technology of China, Hefei, 230026, China.
² Center for Biosensors and Bioelectronics, Biodesign Institute, Arizona State University, Tempe, AZ, 85287, USA.

Abstract

Surface plasmon resonance microscopy (SPRM) is a versatile platform for chemical and biological sensing and imaging. Great progress in exploring its applications, ranging from single-molecule sensing to single-cell imaging, has been made. In this Minireview, we introduce the principles and instrumentation of SPRM. We also summarize the broad and exciting applications of SPRM to the analysis of single entities. Finally, we discuss the challenges and limitations associated with SPRM and potential solutions.

Keywords: binding kinetics; label-free methods; sensors; single-cell imaging; surface plasmon resonance.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Biosensing Techniques / methods*
Humans
Microscopy / methods*
Single-Cell Analysis / methods*
Surface Plasmon Resonance / methods*

Abstract

Publication types

MeSH terms

Grants and funding