Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

Sci Rep. 2019 Sep 17;9(1):13483. doi: 10.1038/s41598-019-49891-7.

Abstract

The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent field that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; anti-DENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53 nm/nM and 1 pM, respectively with Kd = 1.02 × 10-10 M.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioengineering
  • Biosensing Techniques*
  • Dendrimers*
  • Dengue / diagnosis*
  • Dengue / virology
  • Dengue Virus
  • Female
  • Humans
  • Male
  • Microscopy, Atomic Force
  • Optical Fibers*
  • Spectrum Analysis, Raman
  • Viral Proteins / analysis

Substances

  • Dendrimers
  • PAMAM Starburst
  • Viral Proteins