The alteration of rainfall pattern under the background of global climate change may affect the quantity and quality of soil dissolved organic matter (DOM). To better understand the responses of soil DOM to rainfall reduction in subtropical forests, we conducted a 6-year rainfall reduction experiment. There were three treatments: control (CK), 30% rainfall reduction (-30%), and 60% rainfall reduction (-60%). With ultraviolet-visible, infrared and three-dimensional fluorescence spectroscopy, we investigated the effects of rainfall reduction on the quantity and structure of DOM from different soil layers of a natural Castanopsis carlesii forest in subtropical China. Rainfall significantly reduced the content of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) in the 0-10 cm soil layer. Specifically, less decreases of DOC than DON were found under the -30% treatment, while more reduction of DOC than DON for the -60% treatment. Under each treatment, the contents of DOC and DON were significantly higher in the 0-10 cm soil layer than that in the 10-20 cm soil layer. Relatively more microbial metabolite with complex structures, such as aromatic humus and alkanes, contributed to soil DOM from the -30% treatment than that from the -60% treatment. Beyond water availability, microbial activity was a key factor regulating the quantity and structure of soil DOM in response to rainfall reduction.
全球气候变化背景下区域降雨格局变化可能深刻影响土壤可溶性有机质(DOM)的数量和质量.为了解亚热带森林土壤DOM对降雨减少的响应,通过6年不同强度(对照、-30%、-60%)的隔离降雨模拟试验,采用光谱技术,研究了降雨减少对亚热带米槠天然林不同深度土壤DOM数量和结构的影响.结果表明: 与对照相比,隔离降雨使0～10 cm土层中可溶性有机碳(DOC)和可溶性有机氮(DON)含量显著降低,其中-30%处理DOC下降幅度小于DON, 而-60%处理DOC下降幅度大于DON,0～10 cm土层中DOC和DON含量都显著高于10～20 cm土层.-30%处理土壤DOM中源于微生物代谢的芳香类腐殖质和烷烃比例上升;-60%处理土壤DOM中微生物代谢产物的相对贡献率减少.除了隔离降雨后水分变化等直接影响外,微生物活性也是本试验区影响DOM数量和结构的重要因素.
Keywords: dissolved organic matter; infrared spectrum; rainfall reduction; three-dimensional fluorescence spectrum; ultraviolet-visible spectrum.