Nitrobenzyl-based fluorescent photocages for spatial and temporal control of signalling lipids in cells

Chem Commun (Camb). 2019 Oct 10;55(82):12288-12291. doi: 10.1039/c9cc05602e.

Abstract

Here we present a set of fluorescent cages prepared by tethering fluorescent dyes to a photolabile group. The developed molecules enable caging of signalling lipids, their delivery to specific cellular membranes, with further imaging, quantification, and controlled photorelease of active lipids in living cells.

MeSH terms

  • Cell Membrane / chemistry
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Lipid Metabolism*
  • Lipids / chemistry*
  • Nitrobenzenes / chemistry*
  • Signal Transduction*

Substances

  • Fluorescent Dyes
  • Lipids
  • Nitrobenzenes