A multi-residue method for GC-MS determination of selected endocrine disrupting chemicals in fish and seafood from European and North African markets

Abstract

An integrated study was conducted to determine the presence of six types of endocrine disrupting chemicals (bisphenol A, triclosan, two alkylphenols, two phenylphenols, eleven organophosphorus pesticides and seven parabens) in the fish and seafood samples from Europe and North Africa. The proposed method involves ultrasound-assisted extraction followed by continuous solid-phase extraction prior to GC-MS analysis. Analytical quality parameters such as linearity, accuracy, precision, sensitivity and selectivity were all good. Limits of detections ranged from 0.5 to 20.0 ng/kg. The relative standard deviation was lower than 7.5% and recoveries ranged from 84 to 105%. The method was successfully used to determine the target analytes in 20 fish and seafood samples from different fish shops and supermarkets in Europe and North Africa. Analyte contents spanned the range 4.6-730 ng/kg and were all below the maximum legally allowed limits. EDCs most frequently found in the samples analysed were dichlorvos, 2-phenylphenol and nonylphenol.

Keywords: Continuous solid-phase extraction; Endocrine disrupting chemicals; Fish and seafood samples; Gas chromatography–mass spectrometry; Ultrasound-assisted extraction.