Multidrug-resistant CTX-M-15-positive Klebsiella pneumoniae ST307 causing urinary tract infection in a dog in Brazil

Luciana Sartori; Fábio P Sellera; Quézia Moura; Brenda Cardoso; Louise Cerdeira; Nilton Lincopan

doi:10.1016/j.jgar.2019.09.003

Multidrug-resistant CTX-M-15-positive Klebsiella pneumoniae ST307 causing urinary tract infection in a dog in Brazil

J Glob Antimicrob Resist. 2019 Dec:19:96-97. doi: 10.1016/j.jgar.2019.09.003. Epub 2019 Sep 11.

Authors

Luciana Sartori¹, Fábio P Sellera², Quézia Moura³, Brenda Cardoso³, Louise Cerdeira¹, Nilton Lincopan⁴

Affiliations

¹ Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
² Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
³ Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
⁴ Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.

PMID: 31520809
DOI: 10.1016/j.jgar.2019.09.003

Abstract

Objectives: Klebsiella pneumoniae sequence type 307 (ST307) has emerged as a new high-risk clone worldwide. The aim of this study was to report the draft genome sequence of a multidrug-resistant CTX-M-15-positive K. pneumoniae strain causing urinary tract infection in a companion animal in Brazil.

Methods: Whole-genome sequencing was performed on an Illumina NextSeq platform and the genome was de novo assembled using SPAdes. Data analysis was performed using online tools from the Center for Genomic Epidemiology.

Results: The genome size of K. pneumoniae strain PRETA was calculated at 5 450 575 bp, containing a total of 5355 protein-coding sequences. The strain was assigned to the high-risk ST307 and carried the clinically relevant bla_CTX-M-15 gene, besides other genes conferring resistance to aminoglycosides, tetracyclines, quinolones, trimethoprim and fosfomycin.

Conclusion: These data offer novel information for comparative genomic analyses in order to track the transmission dynamics and epidemiology of the newly emerging high-risk clone ST307.

Keywords: Companion animal; Draft genome; ESBL; Enterobacteriaceae; Extended-spectrum β-lactamase; High-risk clone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Base Sequence
Brazil
Dog Diseases / microbiology*
Dogs
Drug Resistance, Multiple, Bacterial / drug effects
Drug Resistance, Multiple, Bacterial / genetics
Genome Size
Genome, Bacterial
Klebsiella Infections / microbiology*
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
Multilocus Sequence Typing
Urinary Tract Infections / microbiology*
Virulence Factors / genetics
Whole Genome Sequencing
beta-Lactamases / genetics*
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Virulence Factors
beta-lactamase CTX-M-15
beta-Lactamases