Transplantation of human adipose tissue derived-SVF enhance liver function through high anti-inflammatory property

Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Dec;1864(12):158526. doi: 10.1016/j.bbalip.2019.158526. Epub 2019 Sep 12.

Abstract

Although human adipose tissue-derived stromal vascular fraction (SVF) has been considered a promising source of stem cells, its characteristics relevant to treatment of a damaged liver have not been fully elucidated. In the present study, we sought to characterize the property of human SVF and determine the therapeutic utility of SVF in the liver cirrhosis model. We performed microarray, quantitative (q)-PCR experiments, and in vivo therapeutic assays using a liver cirrhotic mouse model. q-PCR results revealed that hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF)-A, Interleukin (IL)-10 and microRNA (miR)-146 were more highly upregulated in SVF than in human adipose-derived mesenchymal stem cells (ASCs). The SVF culture medium (CM) inhibited the activation of hepatic stellate cells in vitro. Injection of SVF significantly suppressed TAA-induced liver fibrosis and repaired liver function by inhibition of infiltrating inflammatory cells and induction of capillary/hepatocyte regeneration in vivo. Injection of IL-10 siRNA treated SVF cells decreased anti-inflammation and anti-fibrotic effects in TAA-induced mice liver. Our data indicate that SVF show a high anti-inflammatory property for treating fibrotic liver diseases through IL-10 secretion. Therefore, SVF might be a novel therapeutic alternative for the treatment of liver cirrhosis in clinical settings.

Keywords: Anti-inflammation; Interleukin (IL)-10; Liver cirrhosis; Stromal vascular fraction (SVF).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Disease Models, Animal
  • Hepatocyte Growth Factor / analysis
  • Hepatocyte Growth Factor / immunology
  • Humans
  • Inflammation / immunology
  • Inflammation / physiopathology
  • Inflammation / therapy*
  • Interleukin-10 / analysis
  • Interleukin-10 / immunology
  • Liver / immunology
  • Liver / physiopathology
  • Liver Cirrhosis / immunology
  • Liver Cirrhosis / physiopathology
  • Liver Cirrhosis / therapy*
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / immunology*
  • Mice, Inbred NOD
  • Mice, SCID
  • Vascular Endothelial Growth Factor A / analysis
  • Vascular Endothelial Growth Factor A / immunology

Substances

  • Vascular Endothelial Growth Factor A
  • Interleukin-10
  • Hepatocyte Growth Factor