Proteome of cat semen obtained after urethral catheterization

Marzena Mogielnicka-Brzozowska; Sylwia Prochowska; Wojciech Niżański; Mariusz A Bromke; Jerzy Wiśniewski; Beata Olejnik; Anna Kuzborska; Leyland Fraser; Piotr Młynarz; Władysław Kordan

doi:10.1016/j.theriogenology.2019.09.003

Proteome of cat semen obtained after urethral catheterization

Theriogenology. 2020 Jan 1:141:68-81. doi: 10.1016/j.theriogenology.2019.09.003. Epub 2019 Sep 5.

Affiliations

¹ Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-957, Olsztyn, Poland. Electronic address: mmog@uwm.edu.pl.
² Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 49, 50-366, Wrocław, Poland.
³ Department of Medical Biochemistry, Medical University of Wrocław, Chałubińskiego 10, 50-368, Wrocław, Poland.
⁴ Department of Chemistry and Immunochemistry, Medical University of Wrocław, Bujwida 44a, 50-345, Wrocław, Poland.
⁵ Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-957, Olsztyn, Poland.
⁶ Department of Chemistry, Wroclaw University of Technology, 50-370, Wrocław, Poland.

PMID: 31518731
DOI: 10.1016/j.theriogenology.2019.09.003

Abstract

The binding of seminal plasma (SP) proteins by spermatozoa plays an important role in the regulation of sperm epididymal maturation, motility gaining in female reproductive tracts and sperm-egg interaction. The aim of the study was to analyze the SP and sperm extracts proteome of cat (Felis catus) semen. The seminal plasma and spermatozoa were obtained by urethra catheterization from 10 male cats. Proteins were extracted using RIPA buffer and separated by electrophoresis (SDS-PAGE). The gels were analyzed using MultiAnalyst software. The proteins were subsequently analyzed using NanoUPLC-Q-TOF/MS. UniProt database-supported identification resulted in 106 proteins identified in the cat SP and 98 proteins in the extracts of spermatozoa. Based on a gene ontology analysis, dominant molecular functions of feline SP proteins were binding, catalytic, and antioxidant activity (56%, 33%, and 11% of cases, respectively). The molecular functions of sperm extracts proteins were mainly involved in catalytic activity (41%) and binding (23%). The proteins present in both, the SP and spermatozoa's extracts, were: serum albumin (ALB), semenogelin 2 (SEMG 2), clusterin (CLU), lactoferrin (LTF), prostatic acid phosphatase (ACPP), prolactin inducible protein (PIP), negative elongation factor E (NELF-E) and ectonucleotide pyrophosphatase (ENPP3). Protein-protein interactions analysis showed significant connection for 12 proteins in the cat semen. The seminal plasma proteins which, with high probability score, participate in important metabolic pathways are: glutathione peroxidases (GPx5 and 6), prostatic acid phosphatase (ACPP), β-hexosaminidase (HEXB), polymeric immunoglobulin receptor (pIgR) and serpin family F member 1 (SERPINF1). For sperm protein extracts it were: pyruvate dehydrogenase (PDHB), succinate-CoA-ligase (SUCLA2), malate dehydrogenase (MDH2), ATP synthase F1 subunit alpha (ATP5F1A) and tubulin beta (TUBB).

Keywords: Cat; Proteins; Proteomics; Seminal plasma; Spermatozoa.

MeSH terms

Animals
Cats*
Gene Expression Regulation
Male
Proteome / metabolism*
Semen / physiology*
Semen Analysis / veterinary*
Signal Transduction
Spermatozoa / metabolism
Urinary Catheterization / veterinary*

Substances

Proteome