'Ready Mixed', improved nucleic acid amplification assays for the detection of Escherichia coli DNA and RNA

Jonathan S McQuillan; Matthew W Wilson

doi:10.1016/j.mimet.2019.105721

'Ready Mixed', improved nucleic acid amplification assays for the detection of Escherichia coli DNA and RNA

J Microbiol Methods. 2019 Oct:165:105721. doi: 10.1016/j.mimet.2019.105721. Epub 2019 Sep 10.

Authors

Jonathan S McQuillan¹, Matthew W Wilson²

Affiliations

¹ National Oceanography Centre, European Way, Southampton SO14 3ZH, UK. Electronic address: jonmcq@noc.ac.uk.
² National Oceanography Centre, European Way, Southampton SO14 3ZH, UK.

PMID: 31518593
DOI: 10.1016/j.mimet.2019.105721

Abstract

The selective amplification of E. coli nucleic acid sequences could be used for the early warning of faecal contamination in environmental samples. Modified assays for E. coli DNA and RNA markers are presented with improved integrity and performance over existing methods, and demonstrated using 'ready mixed', preserved reagent mixtures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Bacterial / analysis*
Environmental Monitoring / methods*
Escherichia coli / genetics
Escherichia coli / isolation & purification*
Feces / microbiology
Nucleic Acid Amplification Techniques / methods*
RNA, Bacterial / analysis*

Substances

DNA, Bacterial
RNA, Bacterial