Antibacterial activity of silver nanoparticles of different particle size against Vibrio Natriegens

Yaohua Dong; Hongling Zhu; Yuanyuan Shen; Wenting Zhang; Li Zhang

doi:10.1371/journal.pone.0222322

Antibacterial activity of silver nanoparticles of different particle size against Vibrio Natriegens

PLoS One. 2019 Sep 13;14(9):e0222322. doi: 10.1371/journal.pone.0222322. eCollection 2019.

Authors

Yaohua Dong^{1

2}, Hongling Zhu¹, Yuanyuan Shen¹, Wenting Zhang¹, Li Zhang¹

Affiliations

¹ College of Ocean Science and Engineering, Shanghai Maritime University, Shanghai, China.
² School of Mechanical Engineering, Shanghai JiaoTong University, Shanghai, China.

Abstract

In this study, we describe the synthesis and characterization of silver nanoparticles (Ag-NPs) of different sizes and evaluated their antibacterial activity. Particles size and morphology were characterized by transmission electron microscopy. Evaluation of the bacteriostatic effects was performed by ultraviolet-visible spectrophotometry and comet assays. The smaller the particle size of Ag-NPs, the smaller the value of the minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC), indicating the greater the antibacterial activity. The antibacterial activity was determined by the generation of reactive oxygen species (ROS) by bacteria and by bacterial membrane damage. In this study, we determined ROS-induced damage of bacteria caused by Ag-NPs. In conclusion, our findings indicated that Ag-NPs were effective at different particle sizes and concentrations and that the smaller the particle size of Ag-NPs, the greater the antibacterial activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Metal Nanoparticles / administration & dosage*
Microbial Sensitivity Tests / methods
Microscopy, Electron, Transmission / methods
Particle Size
Silver / pharmacology*
Spectrophotometry, Ultraviolet / methods
Vibrio / drug effects*

Substances

Anti-Bacterial Agents
Silver

Supplementary concepts

Vibrio natriegens

Grants and funding

This work was financially sponsored by the Natural Science Foundation of China (No. 51609133), the Chinese Postdoctoral Science Foundation (No. 2017M620153), and Shanghai science and technology commission project (No. 17DZ1202906). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.