The effects of sympathetic activity induced by ice water on blood flow and brachial artery flow-mediated dilatation response in healthy volunteers

Kristian Magnus Gundersen; Christoffer Nyborg; Øyvind Heiberg Sundby; Jonny Hisdal

doi:10.1371/journal.pone.0219814

The effects of sympathetic activity induced by ice water on blood flow and brachial artery flow-mediated dilatation response in healthy volunteers

PLoS One. 2019 Sep 13;14(9):e0219814. doi: 10.1371/journal.pone.0219814. eCollection 2019.

Authors

Kristian Magnus Gundersen^{1

2}, Christoffer Nyborg^{1

2}, Øyvind Heiberg Sundby¹, Jonny Hisdal^{1

2}

Affiliations

¹ Section of Vascular Investigations, Department of Vascular Surgery, Division of Cardiovascular and Pulmonary Diseases, Oslo University Hospital, Oslo, Norway.
² Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.

Abstract

Objective: To investigate the association between sympathetic activity, reactive hyperemia and brachial artery flow-mediated dilation (FMD).

Background: It is claimed that major surgery has an impact on endothelial function, as observed by post-operative reduced brachial artery FMD response. However, another explanation for the observed reduced FMD response post-operatively may be sympathetic stress-induced reduction in blood flow.

Methods: Seventeen healthy volunteers with a median age (25th-75th percentiles) of 23.5 (23-24.8) years were recruited. Participants' brachial blood flow and FMD response were measured (i) during normal non-stress conditions (Normal1); (ii) during exposure to ice water; and (iii) afterwards, under normal non-stress conditions (Normal2). We continuously measured arterial blood pressure (Finometer), heart rate (ECG), skin blood flow of the index finger (laser Doppler), and brachial artery blood flow and diameter (Ultrasound Doppler). Measurements were taken at baseline; before a 5-min suprasystolic forearm occlusion; and following a 3-min post-occlusion, to measure reactive hyperemia and FMD.

Results: Median (25th-75th percentiles) FMD response after exposure to ice water was reduced compared to non-stress conditions [4.9 (2.9-8.4) % during ice water vs. 9.7 (7.6-12.2) % Normal1 and 9.7 (6.4-10.3) % Normal2, P < 0.001]. Blood flow 60 s after cuff-deflation during ice water exposure was significantly reduced to 328 (289-421) mL compared to non-stress conditions (both P < 0.05). No differences were observed between Normal1 [446 (359-506) mL] and Normal2 [455 (365-515) mL] (both P > 0.05). Heart rate significantly increased during ice water exposure [67 (59-69) beats/min)] compared to 55 (49-60) beats/min during Normal1 and 54 (47-60) beats/min during Normal2 (both P < 0.05). MAP did not change during Normal1 [72 (64-84)] or during Normal2 [71 (65-81) mm Hg] (both P > 0.05), but increased to 86 (75-98) mm Hg during ice water exposure (P < 0.05).

Conclusions: Increased sympathetic activity resulted in decreased blood flow and brachial artery FMD response in healthy volunteers, independent of endothelial dysfunction. Future studies should adjust for blood flow when interpreting the FMD response.

Publication types

Research Support, Non-U.S. Gov't

Grants and funding

The study was financially supported by The Research Council of Norway through the Medical Student Research Program at the University of Oslo, specified to the first authors. URL: https://www.forskningsradet.no/en The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.