Effect of piceatannol-rich passion fruit seed extract on human glyoxalase I-mediated cancer cell growth

Takayuki Yamamoto; Akira Sato; Yusuke Takai; Atsushi Yoshimori; Masahiro Umehara; Yoko Ogino; Mana Inada; Nami Shimada; Aya Nishida; Risa Ichida; Ryoko Takasawa; Hiroko Maruki-Uchida; Sadao Mori; Masahiko Sai; Minoru Morita; Sei-Ichi Tanuma

doi:10.1016/j.bbrep.2019.100684

Effect of piceatannol-rich passion fruit seed extract on human glyoxalase I-mediated cancer cell growth

Biochem Biophys Rep. 2019 Aug 30:20:100684. doi: 10.1016/j.bbrep.2019.100684. eCollection 2019 Dec.

Authors

Takayuki Yamamoto¹, Akira Sato², Yusuke Takai², Atsushi Yoshimori³, Masahiro Umehara¹, Yoko Ogino², Mana Inada², Nami Shimada², Aya Nishida², Risa Ichida², Ryoko Takasawa², Hiroko Maruki-Uchida¹, Sadao Mori¹, Masahiko Sai¹, Minoru Morita¹, Sei-Ichi Tanuma^{2

4}

Affiliations

¹ Research and Development Institute, Health Science Research Center, Morinaga and Company Limited, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama, 230-8504, Japan.
² Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
³ Institute for Theoretical Medicine Inc., 26-1, Muraoka-Higashi 2-chome, Fujisawa, Kanagawa, 251-0012, Japan.
⁴ Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.

Abstract

Passion fruit seed extract (PFSE), a product rich in stilbenes such as piceatannol and scirpusin B, has various physiological effects. It is unclear whether PFSE and its stilbene derivatives inhibit cancer cell proliferation via human glyoxalase I (GLO I), the rate-limiting enzyme for detoxification of methylglyoxal. We examined the anticancer effects of PFSE in two types of human cancer cell lines with different GLO I expression levels, NCI-H522 cells (highly-expressed GLO I) and HCT116 cells (lowly-expressed GLO I). PFSE and its stilbenes inhibited GLO I activity. In addition, PFSE and its stilbenes supressed the cancer cell proliferation of NCI-H522 cells more than HCT116 cells. These observations suggest that PFSE can provide a novel anticancer strategy for prevention and treatment.

Keywords: Anticancer; GLO I, glyoxalase I; Glyoxalase I; HPLC, high-performance liquid chromatography; IL-6, interleukin 6; MAPK, mitogen-activated protein kinase; MG, methylglyoxal; PFSE, Passion fruit seed extract; PI3K, phosphoinositide 3-kinase; Passion fruit seed extract; Piceatannol; STAT3, signal transducers and activators of transcription 3; TCA, tricarboxylic acid; mTOR, mammalian target of rapamycin.