Influence of gibberellic acid and different salt concentrations on germination percentage and physiological parameters of oat cultivars

Anju Chauhan; Bassam A AbuAmarah; Atul Kumar; J S Verma; Hamed A Ghramh; Khalid Ali Khan; Mohammad Javed Ansari

doi:10.1016/j.sjbs.2019.04.014

Influence of gibberellic acid and different salt concentrations on germination percentage and physiological parameters of oat cultivars

Saudi J Biol Sci. 2019 Sep;26(6):1298-1304. doi: 10.1016/j.sjbs.2019.04.014. Epub 2019 Apr 18.

Authors

Anju Chauhan¹, Bassam A AbuAmarah², Atul Kumar¹, J S Verma³, Hamed A Ghramh^{4

5

6}, Khalid Ali Khan^{4

5

6}, Mohammad Javed Ansari⁷

Affiliations

¹ School of Sciences, IFTM University, Moradabad 244001, U.P., India.
² Department of Geology and Geophysics, College of Science, King Saud University, Riyadh, Saudi Arabia.
³ Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145, India.
⁴ Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia.
⁵ Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia.
⁶ Biology Department, Faculty of Science, King Khalid University, Abha 61413, Saudi Arabia.
⁷ Department of Botany, Hindu College Moradabad, Uttar Pradesh 2440001, India.

Abstract

Gibberellic acid (GA₃) is one of the plant growth regulators which improve salt tolerance and mitigate the salt stress impact on plants. The extant analysis was carried out to study the effect of GA₃ and different salt concentrations on seed germination and physiological parameters of oat cultivars. Oats is substantially less tolerant to salt than wheat and barley. Experimentation was conducted as factorial with Completely Randomized Block Design with three replicates. Different concentration of NaCl salt ((25, 50, 75 and 100 mM) were used in test control group and 100 and 150 ppm of GA₃ were used in two group by pre-treated (after 24 h of the seed soaking) and plants were analyzed on 15th day. Results indicate that increasing salinity would decrease the germination percentage and growth parameter in three oat cultivars. Quotes data indicating a 13%, 19.9% and 32.48% in cultivars NDO-2, UPO-212 and UPO-94 germination reduction when soil salinity reaches 50 mM. A 36.02%, 47.33% and 56.365 reduction in germination is likely when soil salinity reaches 100 mM respectively same cultivars. Seeds treated with GA₃ significantly promoted the percentage of germination, shoot and root length, total fresh and dry weight of seedling, tissue water content and seedling vigor index by NDO-2 and UPO-212 under different saline concentration. The maximum average of germination and growth parameters were observed from 150 ppm GA₃ treated seeds. But this concentration was significantly inhibited root length in sensitive cultivar UPO-94 at 75 and 100 mM salt as compared to 100 ppm. We observed that, the high concentration of GA₃ was not suitable for sensitive oat cultivars. Because the plant root are the real workforce behind any plants success. Thus, it may be concluding that, GA₃ treatment could curtail the toxic effect of salinity by increasing germination percentage and shoot and root length, total fresh and dry weight, tissue water content and seedling vigor index in tolerant cultivar.

Keywords: Germination; Gibberellic acid; Oat cultivars; Salt concentrations; Seedling vigor index; Seedlings growth.