Sensors' array of aspiration ion mobility spectrometer as a tool for bacteria discrimination

Victor Bocos-Bintintan; C L Paul Thomas; Ileana Andreea Ratiu

doi:10.1016/j.talanta.2019.120233

Sensors' array of aspiration ion mobility spectrometer as a tool for bacteria discrimination

Talanta. 2020 Jan 1:206:120233. doi: 10.1016/j.talanta.2019.120233. Epub 2019 Aug 6.

Authors

Victor Bocos-Bintintan¹, C L Paul Thomas², Ileana Andreea Ratiu³

Affiliations

¹ Faculty of Environmental Science and Engineering, Babeş-Bolyai University, 30 Fântânele, RO-400294, Cluj-Napoca, Romania.
² Department of Chemistry, School of Science, Loughborough University, Epinal Way, Loughborough, LE11 3TU, United Kingdom.
³ Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, 11 Arany Janos, RO-400028, Cluj-Napoca, Romania; Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 7 Gagarina Str., 87-100, Torun, Poland. Electronic address: andreea_ratiu84@yahoo.com.

PMID: 31514847
DOI: 10.1016/j.talanta.2019.120233

Abstract

The possibility of achieving bacterial discrimination using a miniaturized aspiration ion mobility spectrometer model ChemPro-100i (Environics Oy) has been tested by interrogating the headspace air samples above in vitro bacterial cultures of three species - Escherichia coli, Bacillus subtilis and Staphylococcus aureus, respectively. The ChemPro-100i highly integrated seven sensor array, composed of one a-IMS cell, three MOS (metal oxide sensors), one FET (field effect transistor) sensor and two SC (semiconductor) sensors, provided enough analytical information to discriminate between the three bacterial species. Statistical data processing using either principal component analysis (PCA) or partial least squares discriminant analysis (PLS-DA) was accomplished. We concluded that although the data from the aspiration-type ion mobility sensor, with its 16 ion detectors, is absolutely sufficient to discriminate between various bacteria using their volatile compounds' chemical profile, the other six sensors deliver additional, valuable information.

Keywords: Aspiration ion mobility spectrometry; Bacterial profile; Headspace air samples; Multisensory device; Statistical analysis.

MeSH terms

Bacillus subtilis / isolation & purification*
Bacterial Typing Techniques / methods*
Discriminant Analysis
Escherichia coli / isolation & purification*
Ion Mobility Spectrometry / methods*
Least-Squares Analysis
Principal Component Analysis
Staphylococcus aureus / isolation & purification*
Volatile Organic Compounds / analysis

Substances

Volatile Organic Compounds