Abstract
Aim: In this work, mastoparan analog peptides from wasp venom were tested against Candida albicans and safety assays were performed using cell culture and model zebrafish. Materials & methods: Minimal inhibitory concentration was determined and toxicity was performed using human skin keratinocyte and embryo zebrafish. Also, permeation of peptides through embryo chorion was performed. Results: The peptides demonstrated anti-C. albicans activity, with low cytotoxicity and nonteratogenicity in Danio rerio. The compounds had different permeation through chorion, suggesting that this occurs due to modifications in their amino acid sequence. Conclusion: The results showed that the studied peptides can be used as structural study models for novel potential antifungal agents.
Keywords:
antimicrobial peptides; candidiasis; chorion; mass spectrometry; mastoparans; toxicity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antifungal Agents / administration & dosage
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Antifungal Agents / adverse effects
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Antifungal Agents / pharmacology*
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Antifungal Agents / toxicity
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Candida albicans / drug effects*
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Cell Survival / drug effects
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Cells, Cultured
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Drug-Related Side Effects and Adverse Reactions / pathology
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Humans
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Intercellular Signaling Peptides and Proteins / administration & dosage
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Intercellular Signaling Peptides and Proteins / adverse effects
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Intercellular Signaling Peptides and Proteins / pharmacology*
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Intercellular Signaling Peptides and Proteins / toxicity
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Keratinocytes / drug effects
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Microbial Sensitivity Tests
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Peptides / administration & dosage
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Peptides / adverse effects
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Peptides / pharmacology*
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Peptides / toxicity
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Wasp Venoms / administration & dosage
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Wasp Venoms / adverse effects
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Wasp Venoms / pharmacology*
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Wasp Venoms / toxicity
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Zebrafish
Substances
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Antifungal Agents
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Intercellular Signaling Peptides and Proteins
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Peptides
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Wasp Venoms
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mastoparan