We discuss how to realize rigorous single pulse imaging using a fiber mode-locked laser for the purpose of ultrafast interferometric observation of fast varying dynamic objects. Sub-picosecond pulses are readily picked up in synchronization with the camera operation, allocating one pulse per frame, but rigorous ultrashort single pulse imaging is disturbed by the accumulation of amplified spontaneous emission (ASE) over the exposure time of the camera. Here, we propose four distinct methods to eliminate the ASE-accumulated disruption in the ultrashort optical gating by pulse interferometry and then evaluate their merits and limitations individually by experiments. The proposed four methods are referred to respectively as the time averaged phase modulation, unbalanced pulse overlapping, tandem pulse picking, and second harmonic generation.