Electrophoretic deposition of GHK-Cu loaded MSN-chitosan coatings with pH-responsive release of copper and its bioactivity

Abstract

Despite the fact that titanium has been widely applied in the replacement of bone defects, prosthesis failure still occurred because of the lack of adequate bone-bonding ability and the incidence of post-surgery infections. Concentration-dependent effects of therapeutic copper ions (Cu²⁺) for antibacterial and osteogenic activity have been well-established in the field of biomedical application. In this study, we prepared mesoporous silica nanoparticles (MSN) and MSN-COOH with uniform sphere size (~100 nm) and developed multifunctional chitosan coatings loaded with MSN@GHK-Cu (glycyl-L-histidyl-l-lysine-Cu²⁺) as a suitable strategy by electrophoretic deposition (EPD). The microstructure and composition of the coating were comprehensively characterized by using SEM, XRD, FTIR, and TEM, respectively. The functional activity of Cu²⁺ releasing from the surface was dependent on the pH value of the titanium surface. Through the controllable release of Cu²⁺, the coating achieved not only inhibited adhesion of bacteria but also had good cytocompatibility. The coating based on EPD technique could be considered as a promising surface modification approach for the controlled delivery in situ of drug or other biomolecules.

Keywords: Antibacterial; Coating; Electrophoretic deposition; GHK-Cu; MSN.