Comparative assessment of the interface between poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and fish scales in composites: Preparation, characterization, and applications

Abstract

Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) composites containing fish scales (FSs) were prepared and used in the fabrication of three-dimensional printing filaments. Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) and FS were used to improve the compatibility of FS within a PHBV matrix. Mechanical and morphological characterization indicated that improved adhesion between FS and PHBV-g-MA enhanced the tensile strength of the composite compared with that of PHBV/FS. The PHBV-g-MA/FS composites were also more water-resistant than the PHBV/FS composites. Human foreskin fibroblasts (FBs) were seeded on two series of these composites to assess cytocompatibility. FB proliferation was greater on PHBV/FS composites than on PHBV-g-MA/FS composites. Cell-cycle assays with FBs on PHBV/FS and PHBV-g-MA/FS series composites were unaffected. Moreover, FS enhanced the antioxidant and antimicrobial properties of PHBV-g-MA/FS and PHBV/FS composites, demonstrating the potential of PHBV-g-MA/FS and PHBV/FS composites for biomedical material applications.

Keywords: Cytocompatibility; Fish scales; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Processing technologies; Three-dimensional printing filaments.