Genetic and antigenic characteristics of a human influenza C virus clinical isolate

J Med Virol. 2020 Feb;92(2):161-166. doi: 10.1002/jmv.25589. Epub 2019 Sep 23.

Abstract

Unlike influenza A and B viruses that infect humans and cause severe diseases in seasonal epidemics, influenza C virus (ICV) is a ubiquitous childhood pathogen typically causing mild respiratory symptoms. ICV infections are rarely diagnosed and less research has been performed on it despite the virus being capable of causing severe disease in infants. Here we report on the isolation of a human ICV from a child with acute respiratory disease, provisionally designated C/Victoria/2/2012 (C/Vic). The full-length genome sequence and phylogenetic analysis revealed that the hemagglutinin-esterase-fusion (HEF) gene of C/Vic was derived from C/Sao Paulo lineage, while its PB2 and P3 genes evolved separately from all characterized historical ICV isolates. Furthermore, antigenic analysis using the hemagglutination inhibition (HI) assay found that 1947 C/Taylor virus (C/Taylor lineage) was antigenically more divergent from1966 C/Johannesburg (C/Aichi lineage) than from 2012 C/Vic. Structure modeling of the HEF protein identified two mutations in the 170-loop of the HEF protein around the receptor-binding pocket as a possible antigenic determinant responsible for the discrepant HI results. Taken together, results of our studies reveal novel insights into the genetic and antigenic evolution of ICV and provide a framework for further investigation of its molecular determinants of antigenic property and replication.

Keywords: antigenic evolution; genesis; influenza C virus; phylogenetic evolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / genetics*
  • Child
  • Dogs
  • Gammainfluenzavirus / genetics*
  • Gammainfluenzavirus / immunology*
  • Gene Expression Regulation, Viral
  • Genome, Viral
  • Humans
  • Influenza, Human / virology*
  • Madin Darby Canine Kidney Cells
  • Models, Molecular
  • Phylogeny
  • Protein Conformation
  • RNA, Viral / genetics
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Antigens, Viral
  • RNA, Viral
  • Viral Proteins