The overuse of antibiotics in today's society has resulted in high concentrations of pharmaceutical contaminants in the natural environment. In this work, we investigated the surfactant-mediated adsorption of fluoroquinolone (FQ) antibiotics at the gas-liquid interface and their separation using a semi-batch foam fractionation process. FQs being a zwitterionic compound have an affinity to bind with both cationic and anionic surfactants. The adsorption of FQs to the gas-liquid interface was investigated using a cationic cetyltrimethylammonium bromide (CTAB) and anionic sodium dodecyl sulfate (SDS) surfactant. Ciprofloxacin (CF) was chosen as a model FQ antibiotic. The adsorption properties of the surfactant systems and CF were characterized using pendant-drop tensiometry and surface excess analysis. It was found that CF partitions to the gas-liquid interface more readily in the presence of SDS compared to CTAB. This was also corroborated in the foam fractionation experiments. At optimum operating conditions, CF showed a higher removal efficiency with SDS (96.3%) compared to CTAB (52%). In spite of strong molecular interactions between CTAB and CF, the preferential adsorption of DS--CF complexes was far greater than CTA+-CF complexes. At optimized operating conditions, using SDS as the surfactant, other FQs such as norfloxacin, levofloxacin, and ofloxacin were recovered up to 97.9, 91.7, and 96.7%, respectively, with effluent concentration less than 100 nM. Overall, the work demonstrates foam fractionation as a novel and environment-friendly gas-liquid separation technique for the targeted removal of FQ antibiotics from waterbodies.