Downregulation of miR-424 in placenta is associated with severe preeclampsia

Qiuqin Tang; Jing Gui; Xian Wu; Wei Wu

doi:10.1016/j.preghy.2019.05.017

Downregulation of miR-424 in placenta is associated with severe preeclampsia

Pregnancy Hypertens. 2019 Jul:17:109-112. doi: 10.1016/j.preghy.2019.05.017. Epub 2019 May 23.

Authors

Qiuqin Tang¹, Jing Gui¹, Xian Wu², Wei Wu³

Affiliations

¹ Department of Gynecology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China.
² National Toxicology Program Laboratory, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park 27713, NC, USA.
³ State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park 27713, NC, USA. Electronic address: wwu@njmu.edu.cn.

PMID: 31487625
DOI: 10.1016/j.preghy.2019.05.017

Abstract

Previous studies have suggested that altered miRNA expression in the placenta is associated with preeclampsia. The aim of this study was to investigate the expression of miR-424 in placental samples of severe preeclampsia (sPE) and uncomplicated pregnancy patients. miRNA was isolated from placentas obtained from 30 sPE patients and 30 healthy women. Quantitative real-time polymerase chain reaction was used to analyze the expression of miR-424. The prediction of target genes of miR-424 was performed using miRGen database. The function of these target genes was analyzed further by DAVID and Gorilla software. The expression of miR-424 was significantly lower in patients with sPE than in healthy controls. Changed expression of miR-424 in the case of pregnancy-related hypertensive disorders might affect the Wnt signaling pathway. These factors have a strong correlation with the development of PE. Expression of miR-424 in placenta was lower in patients with sPE, suggesting its role in the pathology of sPE.

Keywords: Placenta; Preeclampsia; miR-424; miRNA.

MeSH terms

Adult
Down-Regulation*
Female
Humans
MicroRNAs / metabolism*
Placenta / metabolism*
Pre-Eclampsia / diagnosis*
Pre-Eclampsia / metabolism
Pre-Eclampsia / physiopathology
Pregnancy
Prenatal Diagnosis*
Real-Time Polymerase Chain Reaction
Severity of Illness Index

Substances

MIRN424 microrna, human
MicroRNAs